PPP1A (PPP1CA) Rabbit Monoclonal Antibody [Clone ID: R04-8E4]

CAT#: TA385024

PP1C alpha/beta Rabbit monoclonal Antibody

Product Images

Western blot detection of PPP1CA+PPP1CB in K562,Rat Brain cell lysates using PPP1CA+PPP1CB Rabbit mAb(1:1000 diluted).Predicted band size:38kDa.Observed band size:38kDa.

Specifications

Product Data

• Clone Name: R04-8E4
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/20
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human PPP1CA+PPP1CB
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 38 kDa; Observed MW: 38 kDa
• Gene Name: protein phosphatase 1 catalytic subunit alpha
• Database Link:
  Entrez Gene 5499 Human
  UniProt ID P62136
• Background: Swiss-Prot Acc.P62136.Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).
• Synonyms: MGC1674; MGC15877; PP-1A; PP1A; PPP1A