PRMT1 Rabbit Monoclonal Antibody [Clone ID: R08-3H8]

CAT#: TA385057

PRMT1 Rabbit monoclonal Antibody

Product Images

Western blot detection of PRMT1 in K562,C6 cell lysates using PRMT1 Rabbit mAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.

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  Immunohistochemistry of PRMT1 in paraffin-embedded Human breast cancer tissue using PRMT1 Rabbit mAb at dilution 1/20

Specifications

Product Data

• Clone Name: R08-3H8
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human PRMT1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 43 kDa; Observed MW: 43 kDa
• Gene Name: protein arginine methyltransferase 1
• Database Link:
  Entrez Gene 3276 Human
  UniProt ID Q99873
• Background: Swiss-Prot Acc.Q99873.Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:16879614, PubMed:26876602). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
• Synonyms: ANM1; HCP1; HMT2; HRMT1L2; IR1B4