PSMB8 Mouse Monoclonal Antibody [Clone ID: 3G3-C1-E2]

CAT#: TA385067

Proteasome beta 8 (3G3) Mouse monoclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 mouse mAb with DAB staining.

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  Western blot detection of PSMB8/LMP7 in C6,Hela,Molt4 and Raji cell lysates using PSMB8/LMP7 mouse mAb (1:1000 diluted).Predicted band size:30KDa.Observed band size:23KDa.

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  Western blot analysis using PSMB8 mouse mAb against Hela (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.

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  Immunofluorescence analysis of Hela cells using PSMB8 mouse mAb (green). Blue

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Specifications

Product Data

• Clone Name: 3G3-C1-E2
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/200-1/1000
  ICC: 1/200-1/1000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human PSMB8 expressed in E. Coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Ascitic Fluid
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 30kDa
• Gene Name: proteasome subunit beta 8
• Database Link:
  Entrez Gene 5696 Human
  UniProt ID P28062
• Background: Swiss-Prot Acc.P28062.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
• Synonyms: beta5i; D6S216; D6S216E; LMP7; MGC1491; PSMB5i; RING10; Y2