PSMB8 Rabbit Monoclonal Antibody [Clone ID: R09-1H1]

CAT#: TA385069

Proteasome beta 8 Rabbit monoclonal Antibody

Product Images

Western blot detection of LMP7 in 3T3,Hela cell lysates using LMP7 Rabbit mAb(1:1000 diluted).Predicted band size:30kDa.Observed band size:23kDa.

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  Immunocytochemistry of LMP7(green) in Hela cells using LMP7 Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R09-1H1
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  ICC/IF: 1/20
  IP: 1/20
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human LMP7
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 30 kDa; Observed MW: 23 kDa
• Gene Name: proteasome subunit beta 8
• Database Link:
  Entrez Gene 5696 Human
  UniProt ID P28062
• Background: Swiss-Prot Acc.P28062.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
• Synonyms: beta5i; D6S216; D6S216E; LMP7; MGC1491; PSMB5i; RING10; Y2