Rab5 (RAB5A) Rabbit Monoclonal Antibody [Clone ID: R06-4J3]

CAT#: TA385107

Rab5 Rabbit monoclonal Antibody

Product Images

Western blot detection of Rab5 in K562,C6,3T3,Hela cell lysates using Rab5 Rabbit mAb(1:1000 diluted).Predicted band size:24kDa.Observed band size:24kDa.

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  Immunohistochemistry of Rab5 in paraffin-embedded Human breast cancer tissue using Rab5 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R06-4J3
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Rab5
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 24 kDa; Observed MW: 24 kDa
• Gene Name: RAB5A, member RAS oncogene family
• Database Link:
  Entrez Gene 5868 Human
  UniProt ID P20339
• Background: Swiss-Prot Acc.P20339.The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 .
• Synonyms: RAB5