RAF1 Rabbit Polyclonal Antibody

CAT#: TA385122

Raf1 Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1,Raf-1 Polyclonal Antibody was diluted at 1:2(4°C,overnight). 2, Sodium citrate pH 6. was used for antibody retrieval(>98°C,2min). 3,Secondary antibody was diluted at 1:2(room tempeRature, 3min)

• 

  Immunofluorescence analysis of mouse liver tissue. 1,Raf-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. A:Raf1. B: DAPI. C: A+B

• 

  Western blot analysis of Raf1 in HepG2 cells using Raf1 antibody.

• 

  Western blot analysis of Raf1 in rat liver, using Raf1 Antibody. The lane on the right is blocked with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: IF: 1/50-200
  WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/5000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):75
• Gene Name: Raf-1 proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 5894 Human
  UniProt ID P04049
• Background: Swiss-Prot Acc.P04049.
• Synonyms: C-RAF; CRAF; NS5; RAF; Raf-1