Rb (RB1) Rabbit Polyclonal Antibody

CAT#: TA385138

Rb Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Retinoblastoma Antibody.

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  Immunofluorescent analysis of SH-SY5Y cells, using Retinoblastoma Antibody.

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  Western blot analysis of Retinoblastoma expression in (1) Jurkat cell lysate; (2) MCF-7 cell lysate.

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000-1/2000
  IHC: 1/100-1/500
  ICC/IF: 1/100-1/500
  IP: 1/50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human Retinoblastoma
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 105kDa
• Gene Name: RB transcriptional corepressor 1
• Database Link:
  Entrez Gene 5925 Human
  UniProt ID P06400
• Background: Swiss-Prot Acc.P06400.The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression.
• Synonyms: OSRC; osteosarcoma; p105-Rb; pp110; pRb; RB