Rb2 p130 (RBL2) Rabbit Monoclonal Antibody [Clone ID: R02-5D6]

CAT#: TA385145

Phospho-Rb2 p130 (Ser952) Rabbit monoclonal Antibody

Product Images

Western blot detection of Rb2 p130 (Phospho-Ser952) in K562,Hela cell lysates using Rb2 p130 (Phospho-Ser952) Rabbit mAb(1:1000 diluted).Predicted band size:128kDa.Observed band size:130kDa.

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  Immunocytochemistry of Rb2 p130 (Phospho-Ser952)(green) in Hela cells using Rb2 p130 (Phospho-Ser952) Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of Rb2 p13 (Phospho-Ser952) in paraffin-embedded Human tonsil using Rb2 p13 (Phospho-Ser952) Rabbit mAb at dilution 1/2

Specifications

Product Data

• Clone Name: R02-5D6
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/20-1/100
  ICC/IF: 1/20-1/100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 128 kDa; Observed MW: 130 kDa
• Gene Name: RB transcriptional corepressor like 2
• Database Link:
  Entrez Gene 5934 Human
  UniProt ID Q08999
• Background: Swiss-Prot Acc.Q08999.Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
• Synonyms: FLJ26459; p130; PRB2; Rb2; RBR-2