ROCK1 Rabbit Polyclonal Antibody

CAT#: TA385172

ROCK1 Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody.

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  Immunofluorescent analysis of Hela cells, using ROCK1 Antibody .

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  Western blot analysis of ROCK1 expression in Ramos cell lysate.

Specifications

Product Data

• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  ICC/IF: 1/50-1/200
  IP: 1/50
  FC: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human ROCK1
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 158kDa
• Gene Name: Rho associated coiled-coil containing protein kinase 1
• Database Link:
  Entrez Gene 6093 Human
  UniProt ID Q13464
• Background: Swiss-Prot Acc.Q13464.ROCK1 Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing.
• Synonyms: MGC43611; MGC131603; p160-ROCK; p160ROCK; PRO0435