RUNX2 Rabbit Monoclonal Antibody [Clone ID: R07-9C7]

CAT#: TA385196

RUNX Rabbit monoclonal Antibody

Product Images

Western blot detection of RUNX1/2/3 in RAW264.7 cell lysates using RUNX1/2/3 Rabbit mAb(1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa.

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  Western blot detection of RUNX1/2/3 in Jurkat,Rat Brain,C6,CHO-K1 cell lysates using RUNX1/2/3 Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size:49kDa.

Specifications

Product Data

• Clone Name: R07-9C7
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/2000
  IHC: 1/50
  ICC/IF: 1/50
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human RUNX1/2/3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 49 kDa; Observed MW: 49 kDa
• Gene Name: runt related transcription factor 2
• Database Link:
  Entrez Gene 860 Human
  UniProt ID Q01196
• Background: Swiss-Prot Acc.Q01196.CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells .
• Synonyms: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; MGC120022; MGC120023; OSF-2; OSF2; PEA2aA; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1