SIRP alpha (SIRPA) Rabbit Polyclonal Antibody

CAT#: TA385241

SIRP alpha Rabbit polyclonal Antibody

Product Images

Western Blot analysis of various cells using SIRP-α1 Polyclonal Antibody

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  Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Sirp alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

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  Western blot analysis of lysates from HepG2 cells, using Sirp alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/20000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human Sirp alpha1. AA range:451-500
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):55
• Gene Name: signal regulatory protein alpha
• Database Link:
  Entrez Gene 140885 Human
  UniProt ID P78324
• Background: Swiss-Prot Acc.P78324.Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function . Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
• Synonyms: BIT; CD172A; MFR; MYD-1; MYD1; p84; PTPNS1; SHPS-1; SHPS1; SIRP; SIRP-ALPHA-1; Sirp-alpha-2; Sirp-alpha-3; SIRPalpha; SIRPalpha2