SMAD2 Rabbit Monoclonal Antibody [Clone ID: R03-3F8]

CAT#: TA385266

Smad2 Rabbit monoclonal Antibody

Product Images

Western blot detection of Smad2 in Hela,CHO-K1,Jurkat cell lysates using Smad2 Rabbit mAb(1:500 diluted).Predicted band size:52kDa.Observed band size:52kDa.

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  Immunohistochemistry of Smad2 in paraffin-embedded Human lung cancer tissue using Smad2 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R03-3F8
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  IHC: 1/20
  ICC/IF: 1/20-1/100
  IP: 1/20
• Reactivities: Hamster, Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Smad2
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 52 kDa; Observed MW: 52 kDa
• Gene Name: SMAD family member 2
• Database Link:
  Entrez Gene 4087 Human
  UniProt ID Q15796
• Background: Swiss-Prot Acc.Q15796.Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
• Synonyms: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440