SQSTM1 Rabbit Monoclonal Antibody [Clone ID: R06-9J2]

CAT#: TA385314

SQSTM1/p62 Rabbit monoclonal Antibody

Product Images

Western blot detection of SQSTM1 in K562,3T3,Hela cell lysates using SQSTM1 Rabbit mAb(1:1000 diluted).Predicted band size:48kDa.Observed band size:62kDa.

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  Immunohistochemistry of SQSTM1 in paraffin-embedded Human breast cancer tissue using SQSTM1 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R06-9J2
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/200
  ICC/IF: 1/100
  IP: 1/20
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human SQSTM1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 48 kDa; Observed MW: 62 kDa
• Gene Name: sequestosome 1
• Database Link:
  Entrez Gene 8878 Human
  UniProt ID Q13501
• Background: Swiss-Prot Acc.Q13501.Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:24128730, PubMed:20168092). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD . May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102).
• Synonyms: A170; EBIAP; ORCA; OSIL; p60; p62; p62B; PDB3; ZIP3