SC35 (SRSF2) Rabbit Monoclonal Antibody [Clone ID: R04-7J2]

CAT#: TA385322

SC35 Rabbit monoclonal Antibody

Product Images

Western blot detection of SC35 in C6,MCF7 cell lysates using SC35 Rabbit mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:35KDa.

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  Immunohistochemistry of SC35 in paraffin-embedded Human tonsil using SC35 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R04-7J2
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/200
  IP: 1/20
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human SC35
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 26 kDa; Observed MW: 35 kDa
• Gene Name: serine/arginine-rich splicing factor 2
• Database Link:
  Entrez Gene 6427 Human
  UniProt ID Q01130
• Background: Swiss-Prot Acc.Q01130.Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
• Synonyms: D11Wsu175e; MRF-1; Pr264; SC35; Sfrs2; Sfrs10