STAT3 Rabbit Monoclonal Antibody [Clone ID: R05-7B2]

CAT#: TA385338

Phospho-STAT3 (Ser727) Rabbit monoclonal Antibody

Product Images

Western blot detection of Phospho-STAT3 (Ser727) in Jurkat,C6,Hela cell lysates using Phospho-STAT3 (Ser727) Rabbit mAb(1:1000 diluted).Predicted band size:88kDa.Observed band size:88kDa.

Specifications

Product Data

• Clone Name: R05-7B2
• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/20
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser727 of human STAT3 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 88 kDa; Observed MW: 88 kDa
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  Entrez Gene 6774 Human
  UniProt ID P40763
• Background: Swiss-Prot Acc.P40763.Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation . May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion .
• Synonyms: APRF; FLJ20882; HIES; MGC16063