Topoisomerase I (TOP1) Rabbit Polyclonal Antibody

CAT#: TA385441

Topoisomerase I Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human colon, using TOP1 Antibody.

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  Western blot analysis of TOP1 expression in Jurkat cell lysate.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1/1000-1/2000
  IHC: 1/50-1/200
  ICC/IF: 1/50-1/200
  FC: 1/50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human TOP1
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 91kDa
• Gene Name: topoisomerase (DNA) I
• Database Link:
  Entrez Gene 7150 Human
  UniProt ID P11387
• Background: Swiss-Prot Acc.P11387.Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
• Synonyms: TOPI