Topoisomerase I (TOP1) Rabbit Monoclonal Antibody [Clone ID: R02-2H9]

CAT#: TA385442

Topoisomerase I Rabbit monoclonal Antibody

Product Images

Western blot detection of Topoisomerase I in K562,Rat Brain,C6,3T3,Hela cell lysates using Topoisomerase I Rabbit mAb(1:1000 diluted).Predicted band size:91kDa.Observed band size:91kDa.

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  Immunocytochemistry of Topoisomerase I(green) in Hela cells using Topoisomerase I Rabbit mAb at dilution 1/200, and DAPI(blue)

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  Immunohistochemistry of Topoisomerase I in paraffin-embedded Human colon cancer tissue using Topoisomerase I Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R02-2H9
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/20-1/100
  ICC/IF: 1/20-1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Topoisomerase I
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 91 kDa; Observed MW: 91 kDa
• Gene Name: topoisomerase (DNA) I
• Database Link:
  Entrez Gene 7150 Human
  UniProt ID P11387
• Background: Swiss-Prot Acc.P11387.Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone . Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
• Synonyms: TOPI