Trp53 Rabbit Monoclonal Antibody [Clone ID: R01-2B9]

CAT#: TA385447

p53 Rabbit monoclonal Antibody

Product Images

Western blot detection of p53 in 3T3 cell lysates using p53 Rabbit mAb(1:1000 diluted).Predicted band size:44kDa.Observed band size:53kDa.

Specifications

Product Data

• Clone Name: R01-2B9
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/20
  IP: 1/20
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of mouse p53
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 44 kDa; Observed MW: 53 kDa
• Gene Name: transformation related protein 53
• Database Link:
  Entrez Gene 22059 Mouse
  UniProt ID P02340
• Background: Swiss-Prot Acc.P02340.Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression . Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
• Synonyms: bbl; bfy; bhy; p44; p53; Tp53