Ubiquitin (UBB) Rabbit Monoclonal Antibody [Clone ID: R06-2H7]

CAT#: TA385513

Ubiquitin K48 Rabbit monoclonal Antibody

Product Images

Western blot detection of K-48 Linkage Specific Ubiquitin in 293 cell lysates using K-48 Linkage Specific Ubiquitin Rabbit mAb(1:1000 diluted).

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  Immunohistochemistry of K-48 Linkage Specific Ubiquitin in paraffin-embedded Human colon cancer tissue using K-48 Linkage Specific Ubiquitin Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R06-2H7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000
  IHC: 1/50
  ICC/IF: 1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human K-48 Linkage Specific Ubiquitin
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Refer to figures
• Gene Name: ubiquitin B
• Database Link:
  Entrez Gene 7314 Human
  UniProt ID P0CG47
• Background: Swiss-Prot Acc.P0CG47.Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
• Synonyms: FLJ25987; MGC8385; OTTHUMP00000064960; OTTHUMP00000064961; UBA80; UBCEP1; UBCEP2