VASP Rabbit Polyclonal Antibody

CAT#: TA385536

VASP Rabbit polyclonal Antibody

Product Images

Western Blot analysis of various cells using VASP Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

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  Western blot analysis of lysates from NIH/3T3 cells, treated with forskolin 40μM 30', using VASP Antibody. The lane on the right is blocked with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  IF: 1/200-1/1000
  ELISA: 1/20000
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human VASP. AA range:124-173
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):46/50
• Gene Name: vasodilator-stimulated phosphoprotein
• Database Link:
  Entrez Gene 7408 Human
  UniProt ID P50552
• Background: Swiss-Prot Acc.P50552.Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
• Synonyms: VASP