Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: TA385541

Vinculin Rabbit polyclonal Antibody

Product Images

Western blot detection of Vinculin in Jurkat,COS7,HepG2,U2OS,Hela cell lysates using Vinculin Rabbit pAb(1:1000 diluted).Predicted band size:125kDa.Observed band size:125kDa.

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  ICC/IF: 1/50-1/200
  IP: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human Vinculin
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 125kDa
• Gene Name: vinculin
• Database Link:
  Entrez Gene 7414 Human
  UniProt ID P18206
• Background: Swiss-Prot Acc.P18206.Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail. In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration.
• Synonyms: CMD1W; metavinculin; MVCL; vinculin