Vinculin (VCL) Rabbit Monoclonal Antibody [Clone ID: R04-8F9]

CAT#: TA385542

Vinculin Rabbit monoclonal Antibody

Product Images

Western blot detection of Vinculin in K562,Rat Brain,C6,3T3,Hela cell lysates using Vinculin Rabbit mAb(1:1000 diluted).Predicted band size:124kDa.Observed band size:124kDa.

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  Immunocytochemistry of Vinculin(green) in Hela cells using Vinculin Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R04-8F9
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/2000
  ICC/IF: 1/50
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Vinculin
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 124 kDa; Observed MW: 124 kDa
• Gene Name: vinculin
• Database Link:
  Entrez Gene 7414 Human
  UniProt ID P18206
• Background: Swiss-Prot Acc.P18206.Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
• Synonyms: CMD1W; metavinculin; MVCL; vinculin