Her2 (ERBB2) Rabbit Monoclonal Antibody [Clone ID: 4D5-8 (trastuzumab)]

CAT#: TA385654

Recombinant Anti-erbB-2 (Her-2/neu) (Clone 4D5-8 (trastuzumab))

Product Images

Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody (Ab00103) 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (TA385654). Antigen retreival was achieved by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer (Dako, cat. #X0909). Primary antibody incubation with TA385654 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody (Dako cat#K4009) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 µg/ml.

Specifications

Product Data

• Clone Name: 4D5-8 (trastuzumab)
• Applications: ELISA, FC, IHC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
• Specificity: Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.
  
  This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  Entrez Gene 2064 Human
  UniProt ID P04626
• Synonyms: C-erbB-2; CD340; erbB-2; HER-2; HER-2/neu; HER2; herstatin; NEU; NGL; p185erbB2; TKR1