Cd3e Rabbit Monoclonal Antibody [Clone ID: 500A2]

CAT#: TA385684

Recombinant Anti-CD3 epsilon (Clone 500A2)

Product Images

Flow-cytometry using anti-CD3epsilon antibody 500A2 (Ab00121) Mouse lymphocytes were stained with an anti-CD44 control (Ab00628-23.0, panel A) or the rabbit-chimeric version of 500A2 (TA385684, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 500A2
• Applications: FC
• Reactivities: Mouse
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG2 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: T cell receptor complexes derived from mouse C6VL-BS thymoma cells (C57BL/Ka) .
• Specificity: The 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains.
  
  This antibody binds to murine CD3 epsilon. It partially cross-blocks binding of clone 145-2C11 (Ab00112) indicating that overlapping epitopes are detected by these antibodies.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD3 antigen, epsilon polypeptide
• Database Link:
  Entrez Gene 12501 Mouse
  UniProt ID P22646
• Synonyms: CD3-epsilon; FLJ18683; T3E; TCRE