Insulin Receptor (INSR) Rabbit Monoclonal Antibody [Clone ID: 83-14]

CAT#: TA385742

Recombinant Anti-Insulin Receptor alpha (Clone 83-14)

Product Images

Flow-cytometry using the anti-Insulin Receptor alpha antibody 83-14 (Ab00137) Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (TA385742, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 83-14
• Applications: ELISA, IF, IHC, IP, WB
• Reactivities: Bovine, Human, Sheep, Pig
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunizing mice with IM-9 lymphocytes followed by purified insulin receptor.
• Specificity: Recognises the extracellular domain of the Insulin Receptor alpha. Amino acids 469 - 592 (exon 7 and 8) of Insulin Receptor alpha.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: insulin receptor
• Database Link:
  Entrez Gene 3643 Human
  UniProt ID P06213
• Synonyms: CD220; HHF5; IR