P Glycoprotein (ABCB1) Rabbit Monoclonal Antibody [Clone ID: MRK16]

CAT#: TA385750

Recombinant Anti-P-glycoprotein (Clone MRK16)

Product Images

Flow-cytometry using the anti-P-glycoprotein antibody MRK16 (Ab00143) HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (TA385750, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: MRK16
• Applications: FC, IF, IP
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Drug resistant K-562 cells.
• Specificity: Recognizes a discontinuous extracellular epitope on P-glycoprotein.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: ATP binding cassette subfamily B member 1
• Database Link:
  Entrez Gene 5243 Human
  UniProt ID P08183
• Synonyms: ABC20; CD243; CLCS; GP170; IBD13; MDR1; MGC163296; P-GP; PGY1