CD3E Rabbit Monoclonal Antibody [Clone ID: YTH 12.5]

CAT#: TA385773

Recombinant Anti-CD3 epsilon (Clone YTH 12.5)

Product Images

Western Blot using anti-CD3E antibody YTH 12.5 (Ab00170) Human spleen sample (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 12.5 (TA385773) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for CD3E is 23.1 kDa. TA385773 successfully detected human CD3E.

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  Immunohistochemical staining of human tonsil tissue using anti-CD3E antibody (Ab00170) YTH 12.5 Anti-CD3E staining of paraffin embedded human tissue using the rabbit-chimeric version of YTH 12.5 (TA385773). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer (Dako, cat. #X0909). Primary antibody incubation with TA385773 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody (Dako cat#K4009) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong membrane staining of T-cells at the periphery of the germinal centre and between follicles may be observed. Recommended concentration, 2-4 µg/ml.

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  Flow-cytometry using anti-CD3 antibody YTH 12.5 (Ab00170) Rhesus monkey lymphocytes were stained with an isotype control (Ab00102-23.0, red) or the rabbit-chimeric version of YTH 12.5 (TA385773, blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.

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  Flow-cytometry using the anti-CD3E antibody YTH 12.5 (Ab00170) Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTH 12.5 (TA385773, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

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  Flow-cytometry using anti-CD3 epsilon antibody YTH 12.5 (Ab00170) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of YTH 12.5 (TA385773, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

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Specifications

Product Data

• Clone Name: YTH 12.5
• Applications: IF, IHC, WB
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: Human CD3.
• Specificity: Recognises the epsilon chain of the human CD3 antigen complex on human T cells.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD3e molecule
• Database Link:
  Entrez Gene 916 Human
  UniProt ID P07766
• Synonyms: CD3-epsilon; FLJ18683; T3E; TCRE