IL2 Receptor alpha (IL2RA) Human Monoclonal Antibody [Clone ID: Basiliximab]

CAT#: TA385813

Recombinant Anti-IL-2R alpha (CD25) (Clone Basiliximab)

Product Images

Immunohistochemical staining of rat spleens using anti-CD25 antibody Ab00188 Formalin fixed rat spleen slices were were stained with a TA385813 at 5 µg/ml. The anti-human CD25 antibody Ab00188 also shows some cross-reactivity with rat in IHC.

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  Immunofluorescent staining of rat thymus using anti-CD25 antibody Ab00188 Formaldehyde-fixed rat thymus slices were stained with TA385813 at 5 µg/ml and detected with a FITC-conjugated secondary antibody. Ab00188 shows weak labelling of rat cells by IF.

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  Western Blot using anti-IL2R alpha (CD25) antibody Ab00188 Human thyroid cancer (lane 1), rat spleen (lane 2), mouse lymph node (lane 3), mouse thymus (lane 4) and rat small intestines (lane 5)samples were resolved on a 10% SDS PAGE gel and blots probed with TA385813 at 1 µg/ml before being detected by a secondary antibody.

Specifications

Product Data

• Clone Name: Basiliximab
• Applications: FC, IF, IHC, WB
• Reactivities: Human, Monkey
• Modifications: NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
• Host: Human
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
• Specificity: Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit – binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) – T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
  
  Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1κ constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 2 receptor subunit alpha
• Database Link:
  Entrez Gene 3559 Human
  UniProt ID P01589
• Synonyms: CD25; IDDM10; IL-2-RA; IL2-RA; IL2R; p55; TCGFR