CD33 Rabbit Monoclonal Antibody [Clone ID: hP67.6 (Gemtuzumab)]

CAT#: TA385858

Recombinant Anti-CD33 (Clone hP67.6 (Gemtuzumab))

Product Images

Flow-cytometry using anti-CD33 antibody hP67.6 (Gemtuzumab) (Ab00283) Human leukocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of hP67.6 (Gemtuzumab) (TA385858, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: hP67.6 (Gemtuzumab)
• Applications: FC, IF
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Gemtuzumab was generated by huminization of the murine P67.6 clone, raised against CD33-transfected FMY9S5 cells of human origin. CD33 is a transmembrane receptor expressed on predominantly on cells of the myeloid lineage, but it can also be found on some lymphoid cells.
• Specificity: Recognises human CD33.
  
  A human IgG4 variant conjugated to derivative of the cytotoxic drug calicheamicin gamma 1, N-acetylcalicheamicin dimethyl hydrazide, has been used as a therapeutic against acute myeloid leukemia. This antibody may also be used in flow cytometry and immunofluorescence.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD33 molecule
• Database Link:
  Entrez Gene 945 Human
  UniProt ID P20138
• Synonyms: FLJ00391; gp67; p67; SIGLEC-3; SIGLEC3