FEN1 Rabbit Monoclonal Antibody [Clone ID: SAIC-21C-4]

CAT#: TA385889

Recombinant Anti-FEN1 (Clone SAIC-21C-4)

Product Images

Flow cytometry using the Anti-FEN1 antibody SAIC-21C-4 (Ab00311). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-21C-4 (TA385889, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-FEN1 antibody SAIC-21C-4 (Ab00311). Human colon tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-21C-4 (TA385889) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-21C-4
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: The KLH-cojugated peptide "SIEEIVR", derived from the Flap endonuclease 1 involved in DNA replication and repair.
• Specificity: Recognises human FEN1.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: flap structure-specific endonuclease 1
• Database Link:
  Entrez Gene 2237 Human
  UniProt ID P39748
• Synonyms: FEN-1; hFEN-1; MF1; RAD2