HSP27 (HSPB1) Rabbit Monoclonal Antibody [Clone ID: SAIC-23B-22]

CAT#: TA385891

Recombinant Anti-HSPB1 (Clone SAIC-23B-22)

Product Images

Flow cytometry using the Anti-HSPB1 antibody SAIC-23B-22 (Ab00313). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-23B-22 (TA385891, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-HSPB1 antibody SAIC-23B-22 (Ab00313). Human heart(A) (0.1µg/ml) and human placenta(B) (0.3ug/ml) tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-23B-22 (TA385891), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-23B-22
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Peptide "DGVVEITGK" derived from Heat shock protein beta-1, involved in stress resistance and actin organisation, conjugated to KLH.
• Specificity: Recognises human HSPB1.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  Entrez Gene 3315 Human
  UniProt ID P04792
• Synonyms: CMT2F; DKFZp586P1322; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27