RRM2 Rabbit Monoclonal Antibody [Clone ID: SAIC-30C-18]

CAT#: TA385903

Recombinant Anti-RRM2 (Clone SAIC-30C-18)

Product Images

Flow cytometry using the Anti-RRM2 antibody SAIC-30C-18 (Ab00322). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (TA385903, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-RRM2 antibody SAIC-30C-18 (Ab00322). Jurkat cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (TA385903) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-30C-18
• Applications: ELISA, WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: The KLH-conjugated peptide "IEQEFLTEALPVK" derived from Ribonucleoside-diphosphate reductase subunit M2 (RRM2). RRM2 provides precursors required for DNA synthesis.
• Specificity: Recognises human RRM2.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: ribonucleotide reductase regulatory subunit M2
• Database Link:
  Entrez Gene 6241 Human
  UniProt ID P31350
• Synonyms: R2; RR2; RR2M