Peroxiredoxin 4 (PRDX4) Rabbit Monoclonal Antibody [Clone ID: SAIC-40C-8]

CAT#: TA385912

Recombinant Anti-PRDX4 (Clone SAIC-40C-8)

Product Images

Flow cytometry using the Anti-PRDX4 antibody SAIC-40C-8 (Ab00332). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-40C-8 (TA385912, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-PRDX4 antibody SAIC-40C-8 (Ab00332). HEK293 cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40C-8 (TA385912) at 0.001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-40C-8
• Applications: ELISA, WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Peptide "LVQAFQYTDK" derived from the cell redox-regulator Peroxiredoxin-4, conjugated to KLH.
• Specificity: Recognises human PRDX4.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: peroxiredoxin 4
• Database Link:
  Entrez Gene 10549 Human
  UniProt ID Q13162
• Synonyms: AOE37-2; PRX-4; Prx-IV