UBE2C Rabbit Monoclonal Antibody [Clone ID: SAIC-41A-1]

CAT#: TA385913

Recombinant Anti-UBE2C (Clone SAIC-41A-1)

Product Images

Flow cytometry using the anti-UBE2C antibody SAIC-41A-1 (Ab00333). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (TA385913, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-UBE2C antibody SAIC-41A-1 (Ab00333). Caco-2(A) (0.3µg/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (TA385913) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-41A-1
• Applications: ELISA, WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
• Specificity: Recognises human UBE2C.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: ubiquitin conjugating enzyme E2 C
• Database Link:
  Entrez Gene 11065 Human
  UniProt ID O00762
• Synonyms: dJ447F3.2; UBCH10