Rhodopsin (RHO) Mouse Monoclonal Antibody [Clone ID: Rho 1D4]

CAT#: TA385916

Recombinant Anti-Rhodopsin (Clone Rho 1D4)

Specifications

Product Data

• Clone Name: Rho 1D4
• Applications: ELISA, IF, IHC, IP, WB
• Reactivities: Human, Mouse, Rat, Zebrafish, Cow
• Modifications: This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Bleached bovine rod outer segment (ROS).
• Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
  
  The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: rhodopsin
• Database Link:
  Entrez Gene 6010 Human
  UniProt ID P08100
• Synonyms: CSNBAD1; MGC138309; MGC138311; OPN2; Opsin-2; rhodopsin; RP4