TNF alpha (TNF) Human Monoclonal Antibody [Clone ID: CDP 571 (Humicade)]

CAT#: TA385965

Recombinant Anti-TNF alpha (Clone CDP 571 (Humicade))

Product Images

Immunohistochemical analysis of rat liver using anti-TNFalpha antibody Ab00448 Formalin fixed rat liver slices were stained with a TA385965 at 5 µg/ml.

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  Western Blot using anti-TNFalpha antibody Ab00448 Rat liver (lane 1), rat spinal cord (lane 2), mouse testis (lane 3), rat colon (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with TA385965 at 1 µg/ml before being detected by a secondary antibody. The expected band size for TNFalpha is 26 kDa, however dimers and other higher order complexes are well described in the literature too (c.f. Petersen 1989, PMID: 2583226). TA385965 successfully detected human and rat TNF alpha.

Specifications

Product Data

• Clone Name: CDP 571 (Humicade)
• Applications: ELISA
• Reactivities: Human
• Modifications: NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.
• Host: Human
• Clonality: Monoclonal
• Immunogen: Human TNF-alpha
• Specificity: The antibody binds to human TNF-alpha with a Kd of 0.087 nM.
  
  The antibody binds specifically to TNF-alpha, a pro-inflammatory cytokine which stimulates release of other cytiokines e.g. IL-1, IL-6 and GM-CSF as well as secretion of ICAM-1 and VCAM-1 from endothelial cells. It stimulates acute phase responses and acts as a chemoattractant for NK cells and monocytes/macrophages. The antibody reached Phase II clinical trials with Celltech, UCB.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Synonyms: Cachectin; DIF; TNF-a; TNF-alpha; TNFA; TNFSF2