CD30 (TNFRSF8) Rabbit Monoclonal Antibody [Clone ID: Ki-3]

CAT#: TA385979

Recombinant Anti-CD30 (Clone Ki-3)

Product Images

Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody (Ab00474) Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (TA385979). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer (Dako, cat. #X0909). Primary antibody incubation with TA385979 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody (Dako cat#K4009) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 µg/ml.

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  Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3 (Ab00474) Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (TA385979, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: Ki-3
• Applications: ELISA, FC, IF, IHC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Human CD30.
• Specificity: The antibody binds to CD30.
  
  The antibody binds specifically to CD30, a cell membrane protein which belongs to the tumour necrosis factor receptor family. Incubation of CD30 positive L540 cells with Ki-3 antibody weakly enhances release of soluble CD30 (in contrast to Ki-4, Ab00475). CD30 is expressed on activated lymphocytes, and binding by its ligands TRAF2 and TRAF5 results in the activation of NF-kappaB, thereby regulating gene expression. CD30 is also associated with Hodgkin Lymphoma, and its expression acts as a tumour marker. Targeting the CD30 antigen with specific monoclonal antibodies is a possible therapy for Hodgkin's lymphoma.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: tumor necrosis factor receptor superfamily member 8
• Database Link:
  Entrez Gene 943 Human
  UniProt ID P28908
• Synonyms: CD30; D1S166E; KI-1