CD30 (TNFRSF8) Rabbit Monoclonal Antibody [Clone ID: Ki-4]

CAT#: TA385982

Recombinant Anti-CD30 (Clone Ki-4)

Product Images

Flow-cytometry using anti-CD30 antibody Ki-4 (Ab00475) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Ki-4 (TA385982) before (B) or after anti-CD3/28 activation (C) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: Ki-4
• Applications: ELISA, FC, IF, IHC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Human CD30.
• Specificity: The antibody binds to CD30.
  
  The antibody binds specifically to CD30, a cell membrane protein which belongs to the tumour necrosis factor receptor family. Incubation of CD30 positive L540 cells with Ki-4 antibody completely inhibits release of soluble CD30 (in contrast to Ki-3, Ab00474). CD30 is expressed on activated lymphocytes, and binding by its ligands TRAF2 and TRAF5 results in the activation of NF-kappaB, thereby regulating gene expression. CD30 is also associated with Hodgkin Lymphoma, and its expression acts as a tumour marker. Targeting the CD30 antigen with specific monoclonal antibodies is a possible therapy for Hodgkin's lymphoma.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: tumor necrosis factor receptor superfamily member 8
• Database Link:
  Entrez Gene 943 Human
  UniProt ID P28908
• Synonyms: CD30; D1S166E; KI-1