CD19 Rabbit Monoclonal Antibody [Clone ID: FMC63]

CAT#: TA386015

Recombinant Anti-CD19 (Clone FMC63)

Product Images

Flow-cytometry using anti-CD19 antibody FMC63 (Ab00613) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of FMC63 (TA386015, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: FMC63
• Applications: FC, IF
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Human prolymphocytic leukaemia cell line JVM3.
• Specificity: Binds human CD19.
  
  This antibody binds human CD19, a 95 kDa protein expressed on the B-lymphocyte lineage. Recently it has been incorporated into a CD19 scFv- 41BB- CD3ζ fusion protein-encoding lentiviral vector which was used in combination with a UCART19 cell therapy to cure high risk CD19+ infant acute lymphoblastic leukemia.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD19 molecule
• Database Link:
  Entrez Gene 930 Human
  UniProt ID P15391
• Synonyms: B4; Leu-12; MGC12802