TGF alpha (TGFA) Rabbit Monoclonal Antibody [Clone ID: tAb2]

CAT#: TA386017

Recombinant Anti-TGFalpha (Clone tAb2)

Product Images

Flow-cytometry using the anti-TGF-alpha antibody tAb2 (Ab00614) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (TA386017, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: tAb2
• Applications: ELISA
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Biologically active hemocyanin-coupled human TGFalpha.
• Specificity: tAb2 binds the N-terminal sequence of transforming growth factor alpha, VVSHFND (with residues 4 to 7 being the minimal binding epitope).
  
  Human transforming growth factora (TGFa) consists of 50 amino acid residues with three disulphide bridges. It belongs to the family of epidermal growth factor related peptides and binds to EGFreceptor. It is involved in cell proliferation in healthy cells and is found in various cancer cells. Transfection of Fibroblasts with the TGFa gene induces tumor formation.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: transforming growth factor alpha
• Database Link:
  Entrez Gene 7039 Human
  UniProt ID P01135
• Synonyms: TFGA