CD3E Rabbit Monoclonal Antibody [Clone ID: 12F6]

CAT#: TA386042

Recombinant Anti-CD3 epsilon (Clone 12F6)

Product Images

Flow-cytometry using anti-CD3 antibody 12F6 (Ab00640) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of 12F6 (TA386042, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 12F6
• Applications: FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Human CD3
• Specificity: This antibody binds to human CD3.
  
  This antibody competes for CD3 binding with the therapeutic antibody OKT3 (Ab00122). 12F6 binding to CD3 on human T-cells stimulates the secretion of TNF-alpha, INF-gamma and IL-10. Humanized versions of this antibody are also available.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD3e molecule
• Database Link:
  Entrez Gene 916 Human
  UniProt ID P07766
• Synonyms: CD3-epsilon; FLJ18683; T3E; TCRE