CD52 Rabbit Monoclonal Antibody [Clone ID: HI186]

CAT#: TA386061

Recombinant Anti-CD52 (Clone HI186 )

Product Images

Flow cytometry using the Anti-CD52 antibody HI186 (Ab00706). Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of HI186 (TA386061, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using Anti-CD52 antibody HI186 (Ab00706). Human spleen tissue lysate (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HI186 (TA386061) 1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: HI186
• Applications: FC, IHC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Human tonsil cells.
• Specificity: This antibody binds to human CD52.
  
  This antibody binds to human CD52.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD52 molecule
• Database Link:
  Entrez Gene 1043 Human
  UniProt ID P31358
• Synonyms: CDw52; HE5