Amyloid Precursor Protein (APP) Rabbit Monoclonal Antibody [Clone ID: 60000000000]

CAT#: TA386065

Recombinant Anti-amyloid beta (Clone 6E10)

Product Images

Western Blot using anti-Amyloid beta antibody 6E10 (Ab00714) Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (TA386065) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). TA386065 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.

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  Immunohistochemical staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody (Ab00714) 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (TA386065). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer (Dako, cat. #X0909). Primary antibody incubation with TA386065 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody (Dako cat#K4009) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 µg/ml.

Specifications

Product Data

• Clone Name: 60000000000
• Applications: ELISA, EM, IHC, IP, WB
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Amyloid-beta peptide.
• Specificity: This antibody binds to amino acid residues 1-16 of beta amyloid.
  
  This antibody recognises human amyloid beta and can be used to detect amyloid in ELISAs, WB and tissue sections.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: amyloid beta precursor protein
• Database Link:
  Entrez Gene 351 Human
  UniProt ID P05067
• Synonyms: A4; AAA; ABPP; AD1; APPI; CTFgamma; CVAP; OTTHUMP00000096096; PN-II; PN2; PreA4