CD64 (FCGR1A) Human Monoclonal Antibody [Clone ID: H22]

CAT#: TA386085

Recombinant Anti-CD64 (Clone H22)

Specifications

Product Data

• Clone Name: H22
• Applications: FC
• Reactivities: Human
• Modifications: This chimeric human antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Human
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA).
• Specificity: This immunotoxin is specific for human CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
  
  This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: Fc fragment of IgG receptor Ia
• Database Link:
  Entrez Gene 2209 Human
  UniProt ID P12314
• Synonyms: CD64; CD64A; FCG1; FCGR1; FcRI; FLJ18345; IGFR1