CD40L (CD40LG) Rabbit Monoclonal Antibody [Clone ID: AT161-10]

CAT#: TA386127

Recombinant Anti-CD40L (Clone AT161-10)

Product Images

Flow-cytometry using the anti-CD40L antibody AT161-10 (Ab00873). Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of AT161-10 (TA386127, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: AT161-10
• Applications: ELISA
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising mice with Chinese hamster ovary (CHO) cells expressing human CD40L.
• Specificity: This antibody is specific for human CD40L, and has been screened for specificity using human CD40L-rat CD40 fusion protein
  
  This antibody has been used in an ELISA analysis to quantify serum levels of CD40L in samples from Alzheimer's patients (direct communication with originator, 2017).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD40 ligand
• Database Link:
  Entrez Gene 959 Human
  UniProt ID P29965
• Synonyms: CD40-L; CD40L; CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP