Cd74 Rabbit Monoclonal Antibody [Clone ID: In-1]

CAT#: TA386130

Recombinant Anti-CD74 (Clone In-1)

Product Images

Western Blot using anti-CD74 antibody In-1 (Ab00876) Mouse spleen (A) and mouse lymph node (B) lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of In-1 (TA386130) at 0.0001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD74 is 31.6kDa, and this protein is glycosylated at several residues. TA386130 successfully detected CD74 in mouse spleen and mouse lymph node lysates.

Specifications

Product Data

• Clone Name: In-1
• Applications: FC, IHC, IP
• Reactivities: Mouse
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Mouse lymphocytes of strain A injected into Wistar rats.
• Specificity: This antibody binds to murine CD74.
  
  The In-1 antibody was used to identify CD74 on lymphocytes in mice. It can be used in immunoprecipitation as well as in flow cytometry (Takahashi et al. 2009, PMID: 19413900) and frozen-section IHC (Momburg et al. 1986, PMID: 3079804).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• Database Link:
  Entrez Gene 16149 Mouse
  UniProt ID P04441
• Synonyms: DHLAG; HLA-DR-gamma; HLADG; Ia-GAMMA; Ii; p33