SLC7A5 Rabbit Monoclonal Antibody [Clone ID: EP3-1]

CAT#: TA386155

Recombinant Anti-CD98 (Clone EP3-1)

Product Images

Flow cytometry using the Anti-CD98 antibody EP3-1 (Ab00898). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of EP3-1 (TA386155, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: EP3-1
• Applications: IF, IP
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. CD98-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
• Specificity: Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2, but showed no reactivity to live HeLa cells by IF.
  
  CD98 may be a target for T or B cell immune responses against tumour cells in cancer patients (Itoh 2008).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: solute carrier family 7 member 5
• Database Link:
  Entrez Gene 8140 Human
  UniProt ID Q01650
• Synonyms: 4F2LC; CD98; CD98LC; D16S469E; E16; hLAT1; LAT1; MPE16