IL2 Receptor alpha (IL2RA) Rabbit Monoclonal Antibody [Clone ID: AHT54]

CAT#: TA386159

Recombinant Anti-IL2R (Clone AHT54)

Product Images

Flow cytometry using the Anti-IL2R antibody AHT54 (Ab00902). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of AHT54 (TA386159, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: AHT54
• Applications: ELISA, FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.
• Specificity: AHT54 recognises a determinant close to the IL-2 binding site.
  
  AHT54 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988). AHT54 has also been shown to have anti-proliferative activities, and chimeric AHT54 antibodies with a human contant region have an increased inhibitory effect on proliferation of T lymphocytes (Rose 1992).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 2 receptor subunit alpha
• Database Link:
  Entrez Gene 3559 Human
  UniProt ID P01589
• Synonyms: CD25; IDDM10; IL-2-RA; IL2-RA; IL2R; p55; TCGFR