CLECSF6 (CLEC4A) Rabbit Monoclonal Antibody [Clone ID: 900000000]

CAT#: TA386167

Recombinant Anti-DCIR (Clone 9E8)

Product Images

Flow-cytometry using the anti-DCIR antibody 9E8 (Ab00911). Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 9E8 (TA386167, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 900000000
• Applications: FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising BALB/c mice with a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.
• Specificity: This antibody is specific for human DCIR, which is expressed by monocytes, B cells, neutrophils, granulocytes and dendritic cells.
  
  This antibody has been used in flow cytometric analysis of DCIR expression by peripheral blood mononuclear cells (Klechevsky et al, 2010). A recombinant humanised form of this antibody fused to protein antigens is under investigation as an antigen-targeting vaccine (Flamar et al, 2012), and has been shown to trigger antigen-specific CD8+ T cell immunity.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: C-type lectin domain family 4 member A
• Database Link:
  Entrez Gene 50856 Human
  UniProt ID Q9UMR7
• Synonyms: CLECSF6; DCIR; DDB27; HDCGC13P; LLIR