Serotonin transporter (SLC6A4) Rabbit Monoclonal Antibody [Clone ID: 8B6]

CAT#: TA386173

Recombinant Anti-SERT (Clone 8B6)

Product Images

Flow cytometry using the Anti-SERT antibody 8B6 (Ab00943). Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 8B6 (TA386173, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 8B6
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: 8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
• Specificity: The 8B6 Fab binds to extracellular loops 2 and 4 of hSERT, as shown by crystallography (Coleman et al, 2016).
  
  The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: solute carrier family 6 member 4
• Database Link:
  Entrez Gene 6532 Human
  UniProt ID P31645
• Synonyms: 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; OTTHUMP00000163633; SERT